IL-37b, Recombinant, Human, aa46-218 (Interleukin 37 Isoform B, IL-1F7b)

Human Interleukin 1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family. IL-1F7 shares ~21%, 24%, and 30% aa sequence identity with mature IL-1alpha, IL-1beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. Five isoforms (IL-1F7a through e) are generated through alternative exon usage and have distinct expression profiles (1-5). Like IL-1alpha, IL-1beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript is the basis for the product offered here. This product, also referred to as IL-1F7b, or IL-1F7 isoform 1, is normally generated from a 218 amino acid (aa) proprotein that consists of a 45aa propeptide plus a 173 aa mature region that is cleaved by caspase-1 to generate the 32kD mature form (aa46-218) (6). IL-1F7 is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (7). Experimental overexpression of IL-1F7b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (7, 8). Both unprocessed and mature IL-1F7b can form homodimers in solution (6, 7). Although IL-1F7b will bind to IL-18 Ralpha with low affinity, this has no effect on IL-18 receptor activity (4, 6, 9). Alternatively, IL-1F7b will also bind to IL-18 BP, generating a complex that interacts with IL-18Rbeta. This has the effect of attenuating IL-18 activity via the IL-18 receptor (9). IL-1F7b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-1F7b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (10).\n\nSource:\nRecombinant protein corresponding to aa46-218 from human IL-37b/IL-1F7b, E. coliпїЅderived.\n\nMolecular Weight: \n~25kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145805

Size

25ug

Form

Supplied as a lyophilized powder in PBS, DTT. BSA free. Reconstitute in 200ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Smith, D.E. et al. (2000) J. Biol Chem 275:1169. 2. Kumar S. et al. (2000) J. Biol Chem 275:10308. 3. Busfield S.J. et al. (2000) Genomics 66:213. 4. Pan, G. et al. (2000) Cytokine 13:1. 5. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127. 6. Kumar S. et al. (2002) Cytokine 18:61. 7. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014. 8. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711. 9. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723. 10. Sharma, S. et al. (2008) J. Immunol. 180:5477.