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IL-6, Recombinant, Human, aa29-212 (Interleukin 6, IL-6 GMP, BSF-2, IFN-beta 2, MGI-2A)

Cat no: 145708

IL-6, Recombinant, Human, aa29-212 (Interleukin 6, IL-6 GMP, BSF-2, IFN-beta 2, MGI-2A)

Interleukin 6 (IL-6), also known as interferon-beta 2, 26kD protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic alpha-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22kD-28kD phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.\nIL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor. \n\nSource:\nRecombinant protein corresponding to aa29-212 from human IL-6 GMP, expressed in E. coli.\n\nMolecular Weight:\n~20-21kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145708

Size

10ug

Form

Supplied as a lyophilized powder in PBS, NaCl. BSA free. Reconstitute in 100-200ug/ml sterile PBS.

Purity

~97% (SDS-PAGE)

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