IL-7 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Interleukin 7 in cell culture supernates, serum and plasma (EDTA).\n\nIL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4 . Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6?7?8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.10\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nA8K673\n\nEntrez Gene ID:\n3574\n\nKit Components:\n96-well plate pre-coated with anti-IL-7, Human\nLyophilized recombinant IL-7, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143933

Size

96Tests

References

1. Heufler C, Topar G, Grasseger A, et al. (September 1993). \"Interleukin 7 is produced by murine and human keratinocytes\". J. Exp. Med. 178 (3): 1109