IL-7 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse IL-7 in cell culture supernates, serum and plasma (EDTA).\n\nInterleukin 7 (IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7 (IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17 (T(H)17) cells in experimental autoimmune encephalomyelitis (EAE).2\n\nAssay Range:\n15.6pg/ml-1000pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP10168\n\nEntrez Gene ID:\n16196\n\nKit Components:\n96-well plate pre-coated with anti-IL-7, Mouse\nLyophilized recombinant IL-7, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143934

Size

96Tests

References

1. Sutherland, G. R., Baker, E., Fernandez, K. E. W., Callen, D. F., Goodwin, R. G., Lupton, S., Namen, A. E., Shannon, M. F., Vadas, M. A. The gene for human interleukin 7 (IL7) is at 8q12-13. Hum. Genet. 82: 371-372, 1989.\n2. Liu, X., Leung, S., Wang, C., Tan, Z., Wang, J., Guo, T. B., Fang, L., Zhao, Y., Wan, B., Qin, X., Lu, L., Li, R., Pan, H., \nSong, M., Liu, A., Hong, J., Lu, H., Zhang, J. Z. Crucial role of interleukin-7 in T helper type 17 survival and expansion in \nautoimmune disease. Nature Med. 16: 191-197, 2010. [PubMed: 20062065\n