IL-8 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-8 in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nInterleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.\n\nAssay Range:\n15.6pg/ml-1000pg/ml(cell culture supernates)\n7.8pg/ml-500pg/ml( serum, plasma)\n\n(body fluids, tissue lysates or cell culture supernates)\n7.8pg/ml-500pg/ml\n(human serum, plasma\n\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP10145\n\nEntrez Gene ID:\n3576\n\nKit Components:\n96-well plate pre-coated with anti-IL-8, Human\nLyophilized recombinant IL-8, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143935

Size

96Tests

References

1. Modi, W. S.; Dean, M.; Seuanez, H. N.; Mukaida, N.; Matsushima, K.; O'Brien, S. J. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum. Genet. 84: 185-187, 1990. \n2. Bruun, J. M.; Pedersen, S. B.; Richelsen, B. Regulation of interleukin 8 production and gene expression in human adipose tissue in vitro. J. Clin. Endocr. Metab. 86: 1267-1273, 2001. \n