IL12A (Interleukin-12 Subunit alpha, IL-12A, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35)

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-g and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent. Human and mouse IL-12 share 70-60aa sequence identity in their p40 and p35 subunits, respectively.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8434-29M

Size

100ug

Applications

IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2A

Additional Info

Recognizes mouse IL12A. Species crossreactivity: recombinant mouse IL-12 p35+p40 heterodimer, recombinant rat IL-12 p35 (50%). Does not react with recombinant human IL-12 p35, recombinant mouse IL-12 p40, IL-23 p19+p40 heterodimer, recombinant canine IL-12 p35+p40 heterodimer, recombinant feline IL-12 p35+p40 heterodimer.