The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 136aa chains, secreted by activated T-cells that act on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as homodimers. IL-17A exhibits cross-species bioactivity between human and murine cells. Recombinant human IL-17A is a 31kD disulfide-linked homodimer of two 136aa polypeptide chains. Manufactured using all non-animal reagents.
Source:
Recombinant protein corresponding to human IL-17A, expressed in E.coli.
Biological Activity:
Assay 1: Determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblast. The expected ED50 is 2ng/ml, corresponding to a specific activity of 5x10e5units/mg.
Assay 2: Measured by its ability to induce IL-6 production by NHDF cells.
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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