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IL21, Recombinant, Human (Interleukin-21, IL-21, Za11, Interleukin21)

Cat no: 149748


Supplier: United States Biological
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IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (gc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant human IL-21 is a 15kD protein consisting of 133aa. Manufactured using all non-animal reagents. Source: Recombinant protein corresponding to human IL-21, expressed in E.coli. Biological Activity: Assay 1: Determined by its ability to proliferate activated B cells. Assay 2: Determined by its ability to induce human CD40L-activated naive B cells to undergo Ig isotype switching to IgG, using an IL-21 concentration of 50ng/ml. Maximal activity was achieved after approximately five cell divisions Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 149748
Size: 10ug
Form: Supplied as a lyophilized powder.
Purity: ~98% (SDS-PAGE and HPLC)
References: 1. Avery, D.T. et. al. (2008) J. Immunol. 181:1767-1779.

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