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IL23A, Mouse, BioAssay(TM) ELISA Kit (Interleukin-23 Subunit Alpha, IL-23 Subunit Alpha, IL-23-A, Interleukin-23 Subunit p19, IL-23p19, SGRF)

Cat no: 138852

IL23A, Mouse, BioAssay(TM) ELISA Kit (Interleukin-23 Subunit Alpha, IL-23 Subunit Alpha, IL-23-A, Interleukin-23 Subunit p19, IL-23p19, SGRF)

The mouse Interleukin-23 subunit alpha (IL-23-A) BioAssay(TM) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse IL-23-A antibody pre-coated onto the 96-well strip plates provided in the kit. Test samples and standards of known IL-23-A concentration are added to these wells and allowed to complex with the bound IL-23-A antibody. A biotinylated mouse monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23-A present in the samples. \n\nIntended Use:\nThe purpose of this kit is the in-vitro quantitative determination of mouse IL-23-A in samples such as sera, plasma, tissue lysates and cell culture supernates. This BioAssay(TM) kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.\n\nSensitivity:\n<2pg/ml\n\nRange:\n31.2pg/ml-2000pg/ml\n\nKit Components:\nPre-coated strip plates, 2x96wells\nProtein standards\nDetection reagents\nSubstrate buffer\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138852

Size

2Plates

Applications

ELISA

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