IL6ST (Interleukin-6 Receptor Subunit beta, IL-6 Receptor Subunit beta, IL-6R Subunit beta, IL-6R-beta, IL-6RB, Interleukin-6 Signal Transducer, Membrane Glycoprotein 130, gp130, Oncostatin-M Receptor Subunit alpha, CD130)

Glycoprotein 130 is a ubiquitously expressed, 130kD type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane. Rat gp130 is synthesized as a 918aa precursor with a 22aa signal sequence, a 596aa extracellular domain (ECD), a 22aa transmembrane region, and a 278aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD. The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains. The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family. Rat gp130 shares 88% and 79% aa sequence identity with mouse and human gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1, IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8428-39R

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wang, Y. et al. (1992) Genomics 14:666. 2. Muller-Newen, G. (2003) Sci. STKE pe40. 3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1. 4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986. 5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.

Additional Info

Recognizes rat GP130. Species Crossreactivity: Recombinant mouse gp130 (~45%), recombinant human gp130 (~6%).