IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant rat IL-7 is a 15kD protein containing 130aa.
Source:
Recombinant protein corresponding to rat IL-7, expressed in E.coli.
Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells. The ED50 was found to be <2ng/ml
Specific Activity:
>5x10e6 units/mg
Endotoxin:
<0.1ng/ug (1EU/ug)
AA Sequence:
MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.