Immunoglobulin-Like Transcript 2 (LT2, Leukocyte Immunoglobulin-like Receptor Subfamily B Member 1, LIR-1, Leukocyte Immunoglobulin-like Receptor 1, CD85 Antigen-like Family Member J, ILT-2, Monocyte/Macrophage Immunoglobulin-like Receptor 7, MIR-7, CD85j

Immunoglobulin-like Transcript 5 is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signalling modulators and inhibitors of cell activation.\n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions:\nFlow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (Frozen): 1:100\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.\n\nPositive Control for Immunohistochemistry:\nHuman tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7502-70H

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Colonna, M. et al. (1999) A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells. J. Leukoc. Biol. 66: 375-381. 2. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J. Immunol. 159: 5192-5196.

Additional Info

Recognizes human Immunoglobulin-like Transcript 2 expressed predominately on B-cells and monocytes, and at lower levels on dendritic cells, T-cells and natural killer (NK) cells.