Immunohistochemistry Detection Kit (Broad Spectrum), Mouse Tissue, BioAssay(TM)

This Immunohistochemistry Detection Kit (Broad Spectrum), Mouse Tissue, BioAssay(TM) is designed to allow immunohistochemical studies of mouse or rat tissue samples using mouse, rabbit, rat and guinea pig primary antibodies. The kit incorporates a highly sensitive horseradish peroxidase (HRP) second antibody to increase sensitivity. Detection system is faster and cleaner because it uses one step instead of two steps, as in the LAB-SA method for the secondary antibody step. After the user-supplied primary antibody is incubated with the sample, an HRP-conjugated, affinity-purified secondary antibody is bound to the primary antibody . A chromogen/substrate system then creates an intense deposit around the antigen/antibody/enzyme complex in the sample.\n\nThe kit incorporates with a high sensitive horseradish peroxidase (HRP) second antibody to increase sensitivity. And the detection system is faster and cleaner because it uses one step of HRP conjugated to second antibody instead of two steps, as in the LAB-SA(3) method. Does not contain biotin or streptavidin, so potential background due to endogenous biotin activity is completely avoided. In addition, a special blocking system is used to eliminate background staining. After a user-supplied primary antibody is incubated with the sample, a HRP-conjugated, affinity-purified secondary antibody is bound to the primary antibody. An AEC chromogen/substrate system then creates an intense red deposit around the antigen/antibody/enzyme complex in the sample.\n\nKit Components:\nI7506-06A: Blocking solution A 1x6ml (ready-to-use). \nI7506-06B: Blocking solution B 1x6ml (ready-to-use). \nI7506-06C: Secondary antibody (HRP) 1x6ml (ready-to-use). \nI7506-06D: AEC 1x6ml. (ready-to-use).\nI7506-06E: Hematoxylin solution 1x6ml (ready-to-use).\nI7506-06F: GVA Mounting 1x10ml (ready-to-use).\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months .\n\nReagents required but not provided:\nXylene, ethanol, and absolute methanol Distilled or deionized water 30% hydrogen peroxide 10mM phosphate-buffered saline (PBS), pH 7.5 (0.05% Tween-20, optional), Primary antibody

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SPECIFICATIONS

Catalog Number

I7506-06

Size

50 Slides

Applications

IHC

Reactivities

Mouse, Rat, GP, Rabbit

References

1. Ching-Hsiang Hsu, et al., Glutathione S-transferase induces murine dermatitis that resembles human atopic dermatitis. Clinical and Experimental Allergy 26(11): 1329-1337 (1996). 2. Gingrich, J., et al., Metastatic Prostate Cancer in a Transgenic Mouse. Cancer Research 56: 4096-4102 (1996). 3. Elias, J.M., et al., Sensitivity and Detection Efficiency of the Peroxidase Antiperoxidase (PAP) Avidin-Biotin Peroxidase Complex (ABC), and Peroxidase-Labeled Avidin-Biotin (LAB) Methods. Am. J. Clin. Pathol. 92: 62-67 (1989). 4. Zuo-Rong Shi, Steven H. Itzkowitz, and Young S. Kim, A comparison of three Immunoperoxidase techniques for antigen detection in colorectal carcinoma tissues. J. Histochem. Cytochem. 36(3): 317-322 (1988).