Inducible Nitric Oxide Synthase, Recombinant, Rat, Western Blot Control (iNOS, iNOS 2, mNOS)

Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.\n

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SPECIFICATIONS

Catalog Number

I0800-09A1

Size

50ul

Applications

WB

Form

Supplied as a liquid in SDS-PAGE sample buffer (reduced).

Purity

Purified

References

1. Lowenstein CJ et al (1992) PNAS 89, 6711, 2. Lyons CR et al (1992) J Biol Chem. 267, 6370, Z3. ie Q-W (1992) Science 256, 225 4. Nunokawa Y et al (1993) BBRC 191, 89