Influenza A, IgG, BioAssay(TM) ELISA Kit

Enzyme immunoassay for the detection and quantitative determination of human IgG antibodies against Influenza A in serum and plasma.\n\nPrinciple of the Test:\nThe Influenza A IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.\n\nKit Components:\nI7649-46A: Coated Microwell Plate (Influenza A strains Beijing and Sydney), 1x96 wells\nI7649-46B: Negative Control (1U/ml), 1x2ml\nI7649-46C: Cut-Off Standard (10U/ml), 1x2ml\nI7649-46D: Weak Positive Control (50U/ml), 1x2ml\nI7649-46E: Positive Control, (150U/ml), 1x2ml\nI7649-46F: IgG (HRP), 1x15ml\nI7649-46G: TMB Substrate, 1x15ml\nI7649-46H: Stop Solution, (0.5M sulfuric acid),1x15ml\nI7649-46J: Sample Diluent, (PBS/BSA buffer, 0.095% sodium azide), 1x60ml\nI7649-46K: Wash Buffer (10X), (PBS, Tween-20), 1x60ml. \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7649-46

Size

1Kit

Applications

ELISA

References

1. Drescher, J., Verhagen, W. Method for determining the equilibrium constant and the concentration of influenza virus IgG antihaemagglutinin antibody molecules by use of EIA titres determined with and without guanidine hydrochloride. J. Virol. Methods, 47: 307-319 (1994). 2. Drescher, J., Verhagen, W. Determination of the concentration of influenza virus antihaemagglutinin antibody molecules of the IgG class and of the equilibrium constant by use of enzyme immunoassay titres determined for graded epitope concentrations. J. Virol. Methods, 55: 257-270 (1995). 3. Lupulescu, E. et al. ELISA in the rapid diagnosis of influenza using as the detecting antibodies polyclonal antinucleoprotein sera. Bacteriol. Virusol. Parazitol. Epidemiol., 41: 63-67 (1996). 4. Marcante, R. et al. Rapid diagnosis of influenza type A infection: comparison of shell-vial culture, directigen flu-A and enzyme-linked immunosorbent assay. New Microbiol., 19:141-147 (1996). 5. Marinich, IG. et al. The immunoprophylaxis of influenza among elderly persons. Zh. Mikrobiol. Epidemiol. Immunobiol. (1997) 3: 60-46. 6. Moldoveanu, Z. et al. Human immune responses to influenza virus vaccines administered by systemic or mucosal routes. Vaccine 13: 1006-1012 (1995). 7. Naikhin, AN. et al. Immuno-enzyme analysis of post-vaccination secretory immunity to influenza A and B viruses using a manufactured monoclonal immunoenzyme test system. Vopr. Virusol. 42: 271-275 (1997).\n8. Naikhin, AN. et al. Monoclonal immuno-enzyme test-system for evaluating secretory immunity to influenza A and B viruses. Vopr. Virusol. 42: 212-216 (1997). 9. Powers, DC. et al. Neuraminidase-specific antibody responses to inactivated influenza virus vaccine in young and elderly adults. Clin. Diagn. Lab. Immunol. 3: 511-516 (1996).