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Insulin (INS) ELISA Kit

Insulin (INS) ELISA Kit

Cat no: ABIN1874279

Supplier: Antibodies-online
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Insulin (INS) ELISA Kit: The microtiter plate provided in this kit has been pre-coated with an antibody specific to insulin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to insulin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain insulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm
Catalogue number: ABIN1874279
Size: 96 tests
Antigen: Insulin (INS)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 125-8000 pg/mL
Sample type: Serum,Plasma,Biological Fluids

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