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Insulin-Like Growth Factor Binding Protein 1 (IGFBP1)

Cat no: I7661-16

Insulin-Like Growth Factor Binding Protein 1 (IGFBP1)

IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) is produced by the liver and regulated by glucocorticoids and insulin at the level of gene transcription. The N-terminal and C-terminal regions are highly homologous among rat, human and bovine sequences. IGFBP-1 inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 detects 10ng SDS-denatured human IGFBP-1.\nImmunoprecipitation: 1:500 precipitates >90% of 10ng IGFBP-1. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-16

Size

50ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Reconstitute with 200ul sterile PBS.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Hill, et al., J. Endocrinol. 122: 87-98 (1989). 2. Elgin, et al., Proc. Nat Acad Sci. USA 84: 3254-3258 (1987). 3. Adashi, et al., Endocrinology 128: 745-762 (1991). 4. Clemmons, et al., Am. J. Physiol. 261: F22 (1991). 5. Clemmons, et al., J. Biol. Chem. 263: 14,203 (1988). 6. Clemmons, et al., J. Clin. Endocrinol. Metab. 67: 1225 (1988).

Additional Info

Recognizes human IGFBP-1 at 30kD. Slight crossreactivity (<1%) with IGFBP-2, -3, -4, -5. Species Crossreactivity: Does not crossreact with bovine, equine, goat or sheep IGFBP-1. Crossreacts weakly with porcine IGFBP-1.

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