Insulin-Like Growth Factor Binding Protein 2 (IGFBP2, BP2, IBP2)

Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. This family contains six members that are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is the second most abundant IGFBP in the circulation and is present in various other biological fluids and tissues of many vertebrate species. Serum IGFBP-2 levels are elevated in many acute or chronic nonphysiological situations, such as shock, fasting, hypoxemia or after traumata, suggesting complex regulation of IGFBP-2 expression. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis.\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested. \nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000 detected IGFBP2 in concentrated T98G serum-free culture supernatant. T98G cell culture supernatant was resolved by electrophoresis, transferred to nitrocellulose and probed with I7661-16G (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.\nImmunoprecipitation: 4ul immunoprecipitated IGFBP2 from 600ul of concentrated T98G serum-free culture supernatant.\nOptimal dilutions determined by the researcher. \nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-16G

Size

200ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Goat, Prc

Form

Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

US Biological application reference: Ludwig, S.K.J. et al, (2012) PLoS ONE 7(12): e52917. doi:10.1371/journal.pone.0052917. 1. Bourner, et al., J. Cell. Biochem. 48: 215-226, 1992. 2. Camacho-Hobner, C., et al., J. Biol. Chem. 267: 11,949-11,956, 1992. 3. Miraki-Mond, F. et al., Cliniical endocrinology 53: 1-11, (2000).

Additional Info

Recognizes bovine IGFBP-2 at 34kD. Crossreactivity: IGFBP-1: < 0.1%, IGFBP-3: < 0.5%, IGFBP-4: < 0.1%, IGFBP-5: < 0.1%. \nSpecies Crossreactivity: human, porcine, goat, equine, mouse, rat and other primate IGFBP-2.