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Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay(TM) ELISA Kit (Rat)

Cat no: 025875

Insulin Like Growth Factor Binding Protein 3 (IGFBP3) BioAssay(TM) ELISA Kit (Rat)

The Rat Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IGFBP3 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\n\nDetection Range:\n12.5-800ng/ml\n\nSensitivity:\n(same/less than)4.6ng/ml\n\nTest Principle:\nThe microtiter plate provided in this kit has been pre-coated with an antibody specific to rat IGFBP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for rat IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

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SPECIFICATIONS

Catalog Number

025875

Size

96Tests

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