Insulin-Like Growth Factor I Receptor (IGF1R, IGFIR, CD221, Clone 1900 Unknown Protein, JTK13, MGC142170, MGC142172)

Insulin-Like Growth Factor I Receptor (IGF-I Receptor) , a 155kD receptor tyrosine kinase, also known as CD221. CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1).\n\nApplications:\nSuitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 1:10; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (paraffin): 1:10-1:40\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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