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Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I, IGF-I, IBP1, Mechano Growth Factor, MGF, Somatomedia C, Somatomedin C)

Cat no: I7661-02D

Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I, IGF-I, IBP1, Mechano Growth Factor, MGF, Somatomedia C, Somatomedin C)

Insulinlike Growth Factor I, is a secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver, which effects a wide range of cells and plays a key role in cell proliferation and growth, inhibition of apoptosis, and is linked with tumor cell growth.\nIGFI signals through binding to the type 1 insulinlike growth factor receptor (IGF1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway. IGFI production is stimulated by growth hormone (GH). and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.\n\nSource:\nRecombinant corresponding to human IGF-I expressed in E.coli. \n\nMolecular Weight: ~7.6kD\n\nActivity: \nThe ED50 was determined by a cell proliferation assay using FDCP1 cells is < 2.0 ng/ml, corresponding to a specific activity of >5 x 105 units/mg.\n\nEndotoxin: <1EU/ug\n\nApplications:\nSuitable for use in Functional Assays. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O to 0.1-1mg/ml. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-02D

Size

100ug

Form

Supplied as a lyophilized powder. Preservative free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Purity

Purity: ~98% (SDS PAGE/HPLC).

Alternative Names

Insulin-like Growth Factor IA, IGF-IA, IGF1A, Insulin-like Growth Factor IB, IGF-IB, IGF1B

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