Insulin-Like Growth Factor II, Recombinant, Human (Insulin-like Growth Factor 2 (Somatomedin A), IGF-II, IGF2, C11orf43, FLJ22066, FLJ44734, PP1446, PP9974, Somatomedin-A)

Source:\nRecombinant corresponding to human Insulin Like Growth Factor-II expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~7.5kD\n\nBiological Activity:\nThe ED50, calculated by the competitive binding of IGF-II plasma derived to human placental membrane is less then 1.0ng/ml, corresponding to a specific activity of 106 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a standard solution of IGF-II as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-14E

Size

50ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~97% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Overexpression of human IGF-II mRNA in the brain of transgenic mice modulates IGFBP-2 gene expression in the medulla oblongata. J Endocrinol 2004 Sep;182(3):445-55. 2. IGF and IGF-binding protein expression in the growth plate of normal, dexamethasone-treated and human IGF-II transgenic mice. J Endocrinol 2002 Oct;175(1):143-53. 3. Kinetics and regulation of site-specific endonucleolytic cleavage of human IGF-II mRNAs. Nucleic Acids Res 2001 Sep 1;29(17):3477-86. 4. A quantitative reverse transcription and polymerase chain reaction assay for human IGF-II allows direct comparison of IGF-II mRNA levels in cancerous breast, bladder, and prostate tissues. Growth Horm IGF Res 2000 Apr;10(2):61-70. 5. Measurement of human IGF-II using Sephacryl extraction: a rapid and reliable assay method. Ann Clin Biochem 1996 May;33 ( Pt 3):201-8. 6. The cis-acting elements involved in endonucleolytic cleavage of the 3' UTR of human IGF-II mRNAs bind a 50 kDa protein. Nucleic Acids Res 1996 Mar 15;24(6):1000-7.