Insulin Receptor (IR)

The insulin receptor is a tetrameric transmembranous glycoprotein composed of two alpha subunits (120kD) and two beta subunits (90kD). The alpha subunits are disulfide linked, totally extracellular and are the insulin binding site. The beta subunits are transmembranous and contain an intracellular tyrosine kinase domain. \nThe biological effects of insulin are mediated primarily through the insulin receptor. The interaction of insulin with the alpha-subunit of the insulin receptor activates the protein tyrosine kinase of the beta-subunit which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adaptor proteins, IRS-1, IRS-2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain-containing signaling proteins.\n\nApplications:\nSuitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7661-22A

Size

100ug

Applications

IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.0. No preservatives added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Soos, et al., Biochem. J. 235: 199 (1986). 2. Brindle, et al., Biochem. J. 268: 615 (1990). 3. Prigent, et al., J. Biol. Chem. 265: 9970 (1990).

Additional Info

Recognizes alpha subunit of human insulin receptor. 90% inhibition of insulin binding adipocytes. Shown to activate receptor Kinase in receptor kinase assay.