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Insulin, Recombinant, Mouse (INS)

Cat no: I7660-07

Insulin, Recombinant, Mouse (INS)

Source:\nRecombinant Mouse from E. coli\n\nPurity:\n(same/more than)95%\n\nEndotoxin:\n1.0EU per 1ug (determined by the LAL method)\n\nFragment:\nThe Insulin composed of Insulin-B (Phe25~Ser54) and the Insulin-A (Gly90~Asn110) linked by a polypeptide linker (GGGGS)\n\nSequence:\nMGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- FVKQHL CGPHLVEALY LVCGERGFFY TPKS GGGGS G IVDQCCTSIC SLYQLENYCN\n\nN-Terminal Tag Sequence:\nMGSSHHHHHHпїЅSSGLVPRGSHпїЅMASMTGGQQMпїЅGRGSEF\n\nEpitope Tag:\nN-terminal Tags: His-tag and T7-tag\n\nMolecular Weight:\n9.9kD\n\nPredicted Isoelectric Point:\n6.7\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation, SDS-PAGE.\nOther applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nyophilized powder may be stored at -20 degrees C and is stable for 12 months at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nNote:\nThermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37 degrees C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.

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SPECIFICATIONS

Catalog Number

I7660-07

Size

10ug

Applications

ELISA, IP, WB

Form

Supplied as a powder lyophilized from a solution containing PBS (pH 7.4) with 0.01% sarcosyl and 5% sucrose.

Purity

(same/more than)95% (SDS-PAGE, reducing).

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