Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210kD member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 aa type I transmembrane glycoprotein that contains a 1113aa extracellular domain (ECD) and a 15aa cytoplasmic tail. The ECD contains one vWFA/I-domain (aa147-360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765-1179. Over aa29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse
Integrin alpha 1.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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