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Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

Cat no: I7661-35Q

Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)

The alpha 5 beta 1 integrin mediates cell adhesion and migration on fibronectin, a glycoprotein critical for normal vertebrate embryonic development. Indirect evidence reported to date suggests that this receptor also functions in the deposition of fibronectin matrices.\n\nSpecificity of the antibody was verified by its ability to immunoprecipitate a5b1 heterodimers from 125-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express a5b1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing condition\n\nApplications: \nInhibition of cell adhesion to fibronectin: 10mg/ml Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete. \nImmunoprecipitation from lysate of 10e6 cell equivalent: 2mg \nFlow cytometry: 10mg/ml Western blot (non-reducing gel) \n\n \n\nStorage and Handling: Maintain frozen at-20 degrees C in undiluted aliquots for up to 12 months.

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SPECIFICATIONS

Catalog Number

I7661-35Q

Size

100ul

Applications

FC, IP, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, containing no preservatives

P Type

Mab

Purity

Ascites

Isotype

IgG3

Additional Info

Recognizes mouse integrin VLA-5 (a5b1), a fibronectin receptor.\nSpecies Crossreactivity: Mouse

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