Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)

CD29 is the integrin B1 chain which is expressed as heterodimer non-covalently associated with the integrin subunits 1 to 6 (CD49a to CD49f) and known as very late antigens (VLA). It is also called platelet GPlla. It has been reported as receptor of ?bronectin. The antigen mediates cellular adhesion primarily to collagen, laminin or fibronectin. It has a broad cellular reactivity but is not expressed by erythrocytes. In normal peripheral blood CD29 blood CD29 is found on approximately 41% of T cells, 40% of CD4+ T lymphocytes, 43% of CD8+ T lymphocytes (1). It is slightly reactive with peripheral B-cells. The co-expression of CD4 and CD29 allows the identi?cation of the helper/inducer subpopulation of CD4 lymphocytes.\n\nApplications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (frozen only). Not assessed for Western Blotting. \n\nRecommended Dilution: \nImmunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-39A

Size

500ul

Applications

FC, IF, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.\nAlso available without BSA and azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Friedlander DR; Zagzag D; Shiff B; Cohen H; Allen JC; Kelly PJ; Grumet M. Migration of brain tumor cells on extracellular matrix proteins in vitro correlates with tumor type and grade and involves alphaV and beta1 integrins. Cancer Research, 1996, 56(8):1939-47.\n2. Hieken TJ; Ronan SG; Farolan M; Shilkaitis AL; Das Gupta TK. Beta 1 integrin expression: a marker of lymphatic metastases in cutaneous malignant melanoma. Anticancer Res, 1996, 16(4B):2321-4.\n3. Lanzafame S; Emmanuele C; Torrisi A. Correlation of alpha 2 beta 1 integrin expression with histological type and hormonal receptor status in breast carcinomas. Pathology, Research and Practice, 1996, 192(10):1031-8.\n4. Price EA; Coombe DR; Murray JC. beta-1 Integrins mediate tumour cell adhesion to quiescent endothelial cells in vitro. British Journal of Cancer, 1996, 74(11):1762-6.\n5. Shiokawa S; Yoshimura Y; Nagamatsu S; Sawa H; Hanashi H; Oda T; Katsumata Y; Koyama N; Nakamura Y. Expression of beta 1 integrins in human endometrial stromal and decidual cells. Journal of Clinical Endocrinology and Metabolism, 1996, 81(4):1533-40.\n6. Marshall JF; Rutherford DC; McCartney AC; Mitjans F; Goodman SL; Hart IR. Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin. Journal of Cell Science, 1995, 108:1227-38.\n7. el Gabalawy H; Wilkins J. Beta 1 (CD29) integrin expression in rheumatoid synovial membranes: an immunohistologic study of distribution patterns. Journal of Rheumatology, 1993, 20(2):231-7.\n8. Marshall JF; Nesbitt SA; Helfrich MH; Horton MA; Polakova K; Hart IR. Integrin expression in human melanoma cell lines: heterogeneity of vitronectin receptor composition and function. International Journal of Cancer, 1991, 49(6):924-31.

Additional Info

Recognizes a non-reduced protein of 110kDa and a reduced protein of 130kDa, identified as CD29 or the integrin b-1 in the family of cell adhesion receptors. Species Reactivity: Human.