Interferon gamma, Recombinant, Mouse (IFNg)

Interferon gamma, Recombinant, Murine (IFNg) is a major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF. \n\nRecombinant protein corresponding to , murine IFNg expressed in E. coli\n\nBiological Activity: Recombinant Murine Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral challenge assay using EMC virus on L929 cells is 1.1-1.3x10e7 Units/mg. The corresponding ED50 is0.04-0.09ng/ml.\n\nAmino Acid Sequence:\nMHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I1000-01

Size

20ug

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O, 0.1% BSA or HSA to 0.1mg/ml.

Purity

(same/more than)95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than)0.1EU/ug.

References

1. Interferon-gamma-oligodendrocyte interactions in the regulation of experimental autoimmune encephalomyelitis. J Neurosci 2007 Feb 21;27(8):2013-24. 2. Role of interferon-gamma in Valpha14+ natural killer T cell-mediated host defense against Streptococcus pneumoniae infection in murine lungs. Microbes Infect 2007 Jan 9. 3. Blood serum interferon-gamma bioactivity is low in weanling mice subjected to acute deficits of energy or both protein and energy. Br J Nutr 2007 Mar;97(3):528-34. 4. Pulmona ry epithelial cells are a source of interferon-gamma in response to Mycobacterium tuberculosis infection. Immunol Cell Biol 2007 Feb 20. 5. Effect of Proinflammatory Cytokines, Tumor Necrosis Factor-alpha and Interferon-gamma on Epithelial Barrier Function and Matrix Metalloproteinase-9 in Madin Darby Canine Kidney Cells. Cell Physiol Biochem 2007;19(1-4):99-112. 6. Inhibition of Intrinsic Interferon-{gamma} Function Prevents Neointima Formation After Balloon Injury. Hypertension 2007 Feb 19.