Interferon gamma, Recombinant, Rat (CHO) (IFNg)

This glycosylated interferon is constitutively produced by Chinese Hamster Ovary (CHO) cells transfected with the chromosomal gamma interferon gene of the rat. Purification is performed from serum-free culture medium by affinity chromatography using monoclonal antibodies to rat gamma interferon. This ultra pure product is intended for laboratory use in in vitro assay systems or in experimental animals. Biological activity has been demonstrated in both rat and murine systems. \n\nThe major advantage of CHO-cell derived recombinant rat gamma interferon over natural rat gamma interferon, is the absence of contaminating lymphokines. This ensures that the effects observed in experimental results can be attributed exclusively to the rat gamma interferon used. \nOne unit of Rat Gamma Interferon approximates the bioactivity contained in one unit of the rat gamma interferon standard prepared by the TNO laboratory. \n \nSource:\nRat, CHO-derived \n \nRecomended Dilution:\nFor dilutions use protein stabilized phosphate buffered saline, pH 8. (Do not use fresh serum). \n \nStorage and Stability:\nLyophilized powder may be stored at-20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml distilled or de-ionized water. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-18B3

Size

10KU

Form

Supplied as a lyophilized powder in PBS, 0.01% gelatin. Reconstitute the vial by injection of 1 ml distilled or de-ionized water (Caution: vial is under vacuum).

Purity

Purified

References

1. van der Meide, P et al; The purification and characterization of rat interferon by use of two monoclonal antibodies. J Gen Virol 1986, 67: 1059 2. Schellekens, H et al; Production and initial characterization of rat interferon. J Gen Virol 1980, 46: 243