Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40kD (p40) subunit and a 35kD (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Kit Components:
1. Capture Antibody, 1x1vial
2. Biotinylated-conjugated Detection Antibody, 1x1vial
Required but not Included:
ELISpot Blue Color Module or equivalent
PBS: 37mM NaCl, 2.7mM KCl, 8.1mM Na2HPO4, 1.5mM KH2PO4, pH 7.2-7.4, 0.2um filtered.
Wash Buffer: 0.05% Tween 20 in PBS
Blocking Buffer: 1% BSA, 5% Sucrose in PBS
Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2um filtered).
37 degrees C CO2 incubator
Deionized H2O
Storage and Stability:
Store components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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