Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8)

Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Interleukin-17 (IL-17) and its cognate receptor, IL- 17R, are examples of cytokine receptor pairs. IL-17 protein exhibits a striking degree of homology with the HSV13 protein which mimics its function.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-2ug/ml\nWestern Blot: 0.1-0.2ug/ml\nFunctional Assays: 0.93-1.3ug/ml\nOptimal working dilutions to be determined by researcher.\n\nRecommended Pair:\nDetection: I8439-04R: Interleukin 17 (Biotin)\nCapture: I8439-07: Interleukin 17\n\nRecommended Secondary Antibodies:\nI1903-30U IgG, H&L, X-adsorbed (HRP)\nI1904-40J: IgG, Fc (HRP) Pab Gt xRb\nI1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8439-07

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4 Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gaffen S.L. Arth. Res. and Therapy 6:240-247 (2004). 2. Parsonage, G. et al. (2008) Prolonged, granulocyte-macrophage colony-stimulating factor-dependent, neutrophil survival following rheumatoid synovial fibroblast activation by IL-17 and TNFalpha. Arthritis Res Ther. 10: R47.

Additional Info

Recognizes human interleukin-17.