Interleukin 4, Recombinant, Mouse (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MGC79402, Pit

Recombinant Mouse IL-4 produced in E. coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13.5kD.\n\nBiological Activity: Interleukin-4 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent prolifiration of Murine HT-2 cells is 2.5ng/ml..\n\nAA Sequence:\nMHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8426-10B

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile ddH2O, 0.1% HSA or BSA to 0.1mg/ml.

Purity

(same/more than)95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than)0.1EU/ug (LAL).

References

1. Evaluation of antibody responses elicited by immunization of mice with a pneumococcal antigen genetically fused to murine HSP70 and murine interleukin-4. Acta Biochim Biophys Sin (Shanghai) 2006 Feb;38(2):129-35. 2. Modified vaccinia virus Ankara immunization protects against lethal challenge with recombinant vaccinia virus expressing murine interleukin-4. J Virol 2004 Nov;78(22):12471-9. 3. Alternative splicing of murine interleukin-4 mRNA. Bull Exp Biol Med 2004 Feb;137(2):179-81. 4. In vivo gene transfer of murine interleukin-4 inhibits colon-26-mediated cancer cachexia in mice. Anticancer Res 2002 Sep-Oct;22(5):2547-54. 5. A murine interleukin-4-Ig fusion protein regulates the expression of Th1- and Th2-specific cytokines in the pancreas of NOD mice. Horm Metab Res 2002 Oct;34(10):561-9. 6. Recombinant herpes simplex virus type 1 expressing murine interleukin-4 is less virulent than wild-type virus in mice. J Virol 2001 Oct;75(19):9029-36