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IQGAP1 (Ras GTPase-activating-like Protein IQGAP1, p195, KIAA0051)

Cat no: I8448-75D

IQGAP1 (Ras GTPase-activating-like Protein IQGAP1, p195, KIAA0051)

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq]\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:16,000\nWestern Blot: 1-3ug/ml, observed in human kidney and mouse kidney lysates on ~200kD band\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I8448-75D

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Ren JG, Li Z, Sacks DB, IQGAP1 integrates Ca2+/calmodulin and B-Raf signaling. The Journal of biological chemistry 2008 Aug 283(34):22972-82.

Additional Info

Recognizes human IQGAP1. Species Crossreactivity: Mouse. Species sequence homology: Rat.

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