Isocitrate Dehydrogenase, Recombinant (IDH1)

Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle. Recombinant MDH is purified by proprietary chromatographic techniques.\n\nUnit Definition: \nOne unit is defined as 1umol of NAD+ production per minute under the assay conditions (25 degrees C, pH 7.5).\n\nStorage and Stability:\nFor long-term storage, add HSA or BSA (0.1% final concentration), aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I8850-01

Size

1mg

Form

Supplied as a liquid in potassium phosphate, 50% glycerol, pH 7.1.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Expression of cytosolic NADP+-dependent isocitrate dehydrogenase in bovine mammary epithelium: Modulation by regulators of differentiation and metabolic effectors.\n\nExp Biol Med (Maywood) 2006 May;231(5):599-610\n\n2. Crystallization and preliminary X-ray crystallographic analysis of yeast NAD+-specific isocitrate dehydrogenase.\n\nActa Crystallograph Sect F Struct Biol Cryst Commun 2005 May 1;61(Pt 5):486-8\n\n3. Substrate-free structure of a monomeric NADP isocitrate dehydrogenase: an open conformation phylogenetic relationship of isocitrate dehydrogenase.\n\nProteins 2006 Apr 1;63(1):100-12\n\n4. Control of isocitrate dehydrogenase catalytic activity by protein phosphorylation in Escherichia coli.\n\nJ Mol Microbiol Biotechnol 2005;9(3-4):132-46\n\n5. The RpoS-mediated regulation of isocitrate dehydrogenase gene expression in Escherichia coli.\n\nCurr Microbiol 2006 Jan;52(1):21-6\n\n6. Analysis of interactions with mitochondrial mRNA using mutant forms of yeast NAD(+)-specific isocitrate dehydrogenase.\n\nBiochemistry 2005 Dec 20;44(50):16776-84