ISOLATE II Blood DNA Kit is designed for the rapid and efficient isolation of highly pure genomic DNA from whole blood, cultured cells, serum, plasma or other body fluids, treated with EDTA, citrate or heparin (Fig. 1), from a variety of different animal species (Fig. 2). It is also possible to purify bacterial and viral DNA (e.g. HBV) from blood samples. For viral DNA, we recommend using cell-free samples (serum or plasma). The kit uses a silica-membrane spin column technology. The isolation process combines fast lysis of the starting material with a solution containing chaotropic ions in the presence of Proteinase K, followed by specific binding of DNA directly to the filter in a spin column. Following simple washing steps, high-quality DNA is eluted under low ionic strength conditions in a slightly alkaline elution buffer. The isolated DNA is suitable for downstream molecular biology applications such as various types of PCR, Southern blotting or any kind of enzymatic reactions.
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