ISOLATE II Plasmid Mini Kit is designed for the rapid and efficient isolation of highly pure plasmid DNA from bacterial cultures using silica-membrane spin column technology. The isolation process combines alkaline lysis of the harvested bacteria, followed by clarification and subsequent specific binding of plasmid DNA directly to the filter of a spin column. Contaminants are efficiently removed during the washing steps. In the final step, highly-pure plasmid DNA is eluted. The kit shows excellent recovery of plasmid DNA and reproducible results (Fig. 1) even from low culture volumes (Fig. 2).
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