ISOLATE II Plant RNA Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of plant tissues, including leaves, bark, roots and fruits. Up to 100mg starting material can be processed per spin column. The protocol is easy to follow on a step-by-step basis. Two highly optimized lysis buffers, containing guanidinium thiocyanate and guanidinium-HCl are provided to ensure lysis for all plant types. The lysis buffers also inactivate RNases, thus protecting the released RNA. The lysate is applied to a spin column to selectively remove contaminating genomic DNA, eliminating the need to perform a separate DNase I digestion step. The RNA is then bound to a silica membrane. Subsequent wash steps remove the remaining cell debris and pure RNA is eluted in the final step with RNase-free water. The isolated RNA shows excellent performance in downstream applications such as real-time PCR (Fig. 1), reverse transcription (Fig. 2), Northern blot analysis, microarrays and RNA protection assays.
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