ITGA11, ITGB1, Recombinant, Human (Integrin alpha 11 beta 1, Integrin alpha-11, MSTP018 / Integrin beta-1, Fibronectin Receptor Subunit beta, VLA-4 Subunit beta, CD29, FNRB, MDF2, MSK12)

Integrin a11B1 is a noncovalent, heterdimeric collagen receptor that contains a 150kD a11 subunit complexed to a 130kD B1/CD29 subunit. a11B1 is the only known a11-containing integrin. By contrast, at least twelve a-integrins share the B1 subunit (1-5). a11B1 is expressed on fibroblasts from cornea, skin and periodontal ligament (3-9). It is also expressed on mesenchymal stem cells and is considered a marker for non-muscle mesenchymal tissue (3, 8-10). Expression of a11B1 is stimulated by TGF-B1, activin A, and type I interferons, and down-regulated by FGF-2 (6, 9-11). The a11 extracellular domain (ECD) contains a vWFA domain which includes the ligand binding site (2-5). The B1 ECD also contains a vWFA domain, which participates in binding. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The dimer assumes a folded configuration at "rest". Divalent cations and intracellular (inside-out) signaling convert it to an active, extended and open conformation (1, 2). The 1120aa human a11 ECD shares 90-93% aa sequence identity with mouse, rat, equine, canine and bovine a11, while the 708aa human B1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline B1. I domain-containing B1 integrins a1B1, a2B1, a10B1 and a11B1 all bind collagens (3-5). a11B1 preferentially binds interstitial collagens such as type I (5, 8, 12). It can contract collagen matrices and influence inflammatory edema (3, 13). Depending on context, a11B1 can support or inhibit cell migration on collagen (7, 8). It is frequently up-regulated in the stroma of lung adenocarcinomas and enhances tumorigenicity (14, 15).\n\nSource: \nRecombinant corresponding to aa21-728 from human B1 at N-terminus and C-terminus expressed in CHO cells.\n\nMolecular Weight: \n~134.6kD (a11), ~88.4kD (B1)\n\nEndotoxin Level:\n(same/less than)1EU/1ug (LAL) \n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. Immobilized Collagen I at 10ug/ml (100ul/well) can bind recombinant human Integrin a11B1 with an apparent KD< 2nM.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-37H

Size

50ug

Form

Supplied as a lyophilized powder in Tris, sodium chloride, magnesium chloride. Reconstitute with PBS to 100ug/ml.

Purity

~95% (SDS-PAGE)

References

1. Takada, Y. et al. (2007) Genome Biol. 8:215. 2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619. 3. Popova, S.N. et al. (2007) Acta Physiol. 190:179. 4. Lehnert, K. et al. (1999) Genomics 60:179. 5. Velling, T. et al. (1999) J. Biol. Chem. 274:25735. 6. Gouttenoire, J. et al. (2010) Eur. J. Cell Biol. 89:307. 7. Popova, S.N. et al. (2004) Dev. Biol. 270:427. 8. Tiger, C-F. et al. (2001) Dev. Biol. 237:116. 9. Carrecedo, S. et al. (2010) J. Biol. Chem. 285:10434. 10. Varas, L. et al. (2007) Stem Cells Dev. 16:965. 11. Coelho, L.F.L. et al. (2006) Cytokine 33:352. 12. Zhang, W-M. et al. (2003) J. Biol. Chem. 278:7270. 13. Svendsen, O.S. et al. (2009) Arterioscler. Thromb. Vasc. Biol. 29:1864. 14. Wang, K-K. et al. (2002) Oncogene 21:7598. 15. Zhu, C-Q. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11754.