Jak1, Active, Recombinant, Human, GST-Tag (Tyrosine-protein Kinase JAK1, Janus Kinase 1, JAK-1, JAK1A, JAK1B)

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.\n\nSource:\nRecombinant corresponding to aa866-1155 from human Jak1, fused to GST-tag at N-terminal, expressed in Sf9 cells.\n\nMolecular Weight:\n~59kD\n\nApplications:\nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nSpecific Activity:\n~1pmol/min/ug\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137306

Size

10ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 16mM glutathione, 20% glycerol.

Purity

Purified (~35%)

References

1. Rodig, S.J., et al. (1998). Cell 93 (3): 373-8. 2. Haan, C., et al. (2001). J. Biol. Chem. 276 (40): 37451-8. 3. Miyazaki, T., et al. (1994). Science 266 (5187): 1045-7.