JAK1 (Janus Kinase 1)

The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity. \n\nApplications:\nSuitable for use in Immunoprecipiation and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 5ug immunoprecipitated JAK1 from 1mg of a human TF1 RIPA lysate as shown in subsequent immunoblot. \nImmunoprecipitation/Western Blot Analysis: 1mg of TF1 cell lysate was immunoprecipitated with 5ug J0900-05. The immunocomplex was subsequently resolved by electrophoresis and transferred to nitrocellulose. Probed with J0900-05 (4ug/ml). Proteins were visualized using the secondary antibody conjugated to HRP and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J0900-05

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplid as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography

Isotype

IgG

References

1. Argetsinger, R., et al., Cell 74: 237 (1993). 2. Witthuhn, B.A., et al., Cell 74: 227-236 (1993). 3. Silvennoinen, O., et al., Science 261: 1736 (1993).

Additional Info

Recognizes human JAK1 at ~130kD and a protein of ~80kD.