JAK2, Mouse, Recombinant (Janus Kinase 2) (Agarose)

The Janus (JAK) family of cytoplasmic tyrosine kinases has been implicated in signal transduction induced by cytokines and growth factors, physically associated with ligand-bound receptors. This association that results in tyrosine phosphorylation and activation. JAK1 is approximately 130kD and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among JAK family members. Family members such as JAK1, JAK2, and TYK2 are ubiquitous, whereas JAK3 is predominantly expressed in T lymphocytes. Studies with mutant cells that do not express JAK1, JAK2, or TYK2 show that their activation is essential for cellular signaling. JAK family kinases may either phosphorylate each other or be phosphorylated by a yet undescribed tyrosine kinase. Different cytokines can activate via phosphorylation distinct JAK family members. In some cells, the membrane-associated gp130 protein has been implicated in the induction of JAK family phosphorylation, although gp130 itself has no known activity.\n\nAutophosphorylation Kinase Assay: 10-20ul was tested in an in vitro autophosphorylation assay. JAK2 enzyme agarose complex was incubated with [g32P]-ATP. Half the reaction mixture was resolved by SDS PAGE and radiolabeled bands were visualized by autoradiography of the gel. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J0901-06

Size

100ul

Applications

IP

Conjugates

Agarose

Form

Supplied as a 50% suspension of Protein A containing the enzyme-antibody complex in 150mM sodium chloride, 50mM Tris-HCl, pH 7.6, 20% glycerol, 0.1mM EDTA, 0.1mM sodium orthovanadate, 50mM NaF, 0.5% NP-40, 1mM dithiothreitol, 0.4mM PMSF, 3ug/ml aprotinin, 2ug/ml leupeptin, 1microug/ml pepstatin A.

Purity

Purified by immunoprecipitation using JAK2 (Janus Kinase 2) Pab Rb xMo (Catalog No: J0901-04) coupled to Protein A agarose.