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JAK2, phosphorylated (Tyr1007) (Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2)

Cat no: J0901-05B

JAK2, phosphorylated (Tyr1007) (Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2)

JAK2 is a member of the Jak kinase family which is ubiquitously expressed. It associates with cytokine receptors and is essential for signal transduction by mediating tyrosine phosphorylation. The protein is composed of four conserved domains, the NH2-terminal FERM domain which is required for interaction with cytokine receptors, the adjacent SH2-like fold with no known function, a kinase-like COOH-terminal JH2 domain that is devoid of enzymatic activity but that inhibits the activity of the COOH-terminal JH1 tyrosine kinase domain. Human JAK2 gene is localized in the chromosomal region 9p24. The protein is highly expressed in spleen, peripheral blood leukocytes, testis, heart and skeletal muscle. Recent studies have implicated de-regulation of JAK2 kinase activity by chromosomal translocations in hematopoietic tumors.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J0901-05B

Size

50ug

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Edward, PF. et al. Molecular and Cellular Biology. 24:4968-4978 (2004). 2. James, NI. et al. Current Opinion in Genetics & Development. 17:8-14 (2007).

Additional Info

Recognizes human JAK2, phosphorylated at Tyr1007. Species Crossreactivity: mouse and rat.

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