JNK1/2, phosphorylated (Thr183, Tyr185), Human (c-Jun NH2-terminal Kinase1/2, SAPK alpha/gamma) BioAssay(TM) ELISA Kit

Sensitivity: \n<0.8units/ml\n\nRange: \n1.6-100units/ml\n\nIncubation Time: \n4 hours\n\nSimple: \nPre-coated strip-well plates. Liquid stable chromogen and stop reagents.\n\nSample size: \n10ul\n\nEconomical: \nOne 96-well plate, plus reagents for 96 determinations. Reagents are stable for multiple runs.\n\nSample type: \nCell extracts/lysates, buffered solutions\n\nPrecision: \nIntra-assay: <10 %.\nInter-assay: <10 %\n\nKit Components:\n*J2500-10H1 Standard 2x1vial\nJ2500-10H2 Standard Diluent Buffer 1x25ml Contains 15 mM sodium azide\nJ2500-10H3 Microtiter Plate 1x96 wells \nJ2500-10H4 JNK1/2 (pTpY183/185) Pab 1x11ml Contains 15 mM sodium azide;J2500-10H 5 IgG Pab (HRP) (100x) 1x125ul Contains 3.3 mM thymol\nJ2500-10H6 HRP Diluent 1x25ml Contains 3.3 mM thymol \nJ2500-10H7 Wash Buffer (25x) 1x100ml\nJ2500-10H78Tetramethylbenzidine (TMB) 1x25ml\nJ2500-10H9 Stop Solution 1x25ml\n\nStorage and Stability:\nStore *J2500-10H1 Standard powder at 4 degrees C liquid at -70 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening.

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SPECIFICATIONS

Catalog Number

J2500-10H

Size

96Tests

Applications

ELISA

References

1. Dong, C., et al. (2001) Signaling by the JNK group of MAP kinases. C-jun N-terminal Kinase. J. Clin. Immunol. 21:253-7. 2. Kuhl, M. (2002) Non-canonical Wnt signaling in Xenopus: regulation of axis formation and gastrulation. Semin. Cell Dev. Biol. 13:243-249. 3. Leppa, S., Bohmann, D. (1999) Diverse functions of JNK signaling and c-Jun in stress response and apoptosis. Oncogene 18:6158-6162. 4. Weston, C.R., et al. (2002) Signal transduction. MAP kinase signaling specificity. Science 296:2345-2347. 5. Davis, R.J. Signal transduction by the JNK group of MAP kinases. Cell 103:239-52. 6. Tournier, C., et al. (2001) MKK7 is an essential component of the JNK signal transduction pathway activated by proinflammatory cytokines. Genes Dev. 15:1419-1426. 7. Chen, N., et al. (2001) Suppression of skin tumorigenesis in c-Jun NH(2)-terminal kinase-2-deficient mice. Cancer Res. 61:3908-3912. 8. Tournier, C., et al. (2000) Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway. Science 88:870-874. 9. Levresse, V., et al. (2002) Regulation of Platinum-Compound Cytotoxicity by the c-Jun N-Terminal Kinase and c-Jun Signaling Pathway in Small-Cell Lung Cancer Cells. Mol. Pharmacol. 262:689-697. 10. Bogoyevitch, M.A., et al. (1995) Cellular stresses differentially activate c-Jun N-terminal protein kinases and extracellular signal-regulated protein kinases in cultured ventricular myocytes. J. Biol. Chem. 270:29710-29717.