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JNK1, active, Recombinant, Mouse (c-Jun N-terminal Kinase 1, JNK, AI849689, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, Mapk8, Prkm8, SAPK1, Stress-activated Protein Kinase JNK1)

Cat no: J2499-94

JNK1, active, Recombinant, Mouse (c-Jun N-terminal Kinase 1, JNK, AI849689, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, Mapk8, Prkm8, SAPK1, Stress-activated Protein Kinase JNK1)

JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at Threonine and Tyrosine residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on S63 and S73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.\n\nSource: \nRecombinant full-length mouse JNK1 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_016700.\n\nMolecular Mass: \nThe approximate molecular weight is 71 kD (see Figure 1 below).\n\nActivity: \nThe specific activity of JNK1 was determined to be 124 nmol/min/mg using an ATF2 substrate\n\nStorage:\nThis product is stable at (same/less than) -70 degrees C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

J2499-94

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

(same/more than) 90% by densitometry

References

1. Derijard, B. et al. (1994) Cell 76:1025.\n2. Kolodziejczyk, S.M. et al. (2001) Curr Biol. 11:1278.

Alternative Names

EC=2.7.11.24

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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