JNK2 alpha1, Recombinant, Human (c-Jun N-terminal Kinase 2, JNK2, JNK2alpha, JNK2A, JNK-55, Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, MAPK9, PRKM9, p54a, p54aSAPK, Stress-activated Protein Kinase 1a, SAPK1a, SAPK, Stress-activated Protein

JNK2 is a soluble 45kD protein (amino acids 1-382) that is activated in response to cellular stress, radiation and growth factors. JNK2 binds to the amino terminal activation domains of c-jun or ATF2 regulating AP-1 transcriptional activity.\n\nUnit Definition: \n~125 units/mg. One unit of JNK2 1 activity is equal to 1 nanomole of phosphate transferred to recombinant ATF-2 fusion protein per minute at 30 degrees C with a final ATP concentration of 100 microM. Recombinant active JNK2 1 is capable of autophosphorylation and also phosphorylates c-Jun at serine 63 and serine 73. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. JNK2 1 is shorter than JNK2 2, and contains a five nucleotide insertion in the C terminal region which causes a shift in the reading frame and subsequent premature termination.\n\nAmino Acid Sequence: \nMSDSKCDSQF YSVQVADSTF TVLKRYQQLK PIGSGAQGIV CAAFDTVLGI NVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLN VFTPQKTLEE FQDVYLVMEL MDANLCQVIH MELDHERMSY LLYQMLCGIKHLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTACTNF MMTPYVVTRY YRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDH IDQWNKVIEQ LGTPSAEFMK KLQPTVRNYV ENRPKYPGIK FEELFPDWIFPSESERDKIK TSQARDLLSK MLVIDPDKRI SVDEALRHPY ITVWYDPAEA EAPPPQIYDA QLEEREHAIEEWKELIYKEV MDWEERSKNG VVKDQPSDAA VSSNATPSQS SSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGS.\n\nStorage: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

J2599-89

Size

2ug

Form

Supplied as a liquid in 50mM Tris, pH 7.5, 0.15M sodium chloride, 0.27M sucrose, 10mM beta-mercaptoethanol, 1mM EDTA and 0.1% Triton X-100.

Purity

(same/more than) 95% (SDS-PAGE). Mass spectrometry of tryptic digest products for identity determination.

References

1. Functional in vivo interactions between JNK1 and JNK2 isoforms in obesity and insulin resistance.\n\nProc Natl Acad Sci U S A 2006 Jul 3;\n\n2. Tumor necrosis factor-induced toxic liver injury results from JNK2-dependent activation of caspase-8 and the mitochondrial death pathway.\n\nJ Biol Chem 2006 Jun 2;281(22):15258-67\n\n3. JNK1 but not JNK2 promotes the development of steatohepatitis in mice.\n\nHepatology 2006 Jan;43(1):163-72\n\n4. MEKK1 controls neurite regrowth after experimental injury by balancing ERK1/2 and JNK2 signaling.\n\nMol Cell Neurosci 2005 Sep;30(1):67-78\n\n5. Cross-talk between JIP3 and JIP1 during glucose deprivation: SEK1-JNK2 and Akt1 act as mediators.\n\nJ Biol Chem 2005 Jul 22;280(29):26845-55\n\n6. Disruption of the Jnk2 (Mapk9) gene reduces destructive insulitis and diabetes in a mouse model of type I diabetes.\n\nProc Natl Acad Sci U S A 2005 May 10;102(19):6931-5