JNK2, Recombinant, Human (c-Jun N-terminal Kinase 2, JNK2alpha, JNK2A, JNK2beta, JNK2B, JNK-55, Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, MAPK9, PRKM9, p54a, p54aSAPK, Stress-activated Protein Kinase 1a, SAPK1a, SAPK, Stress-activated Prot

Recombinant SAPK1/JNK2 (stress-activated protein kinase/c-jun kinase) is a non-glycosilated polypeptide having a molecular mass of 49.5kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nUnit Definition:\n1 U = 1 pmol/min transferred to c-jun (1-169) at 30C\n\nBiological Activity:\nSpecific activity > 300 U/mg. Autophosphorylation < 5%.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J2602

Size

5ug

Form

Supplied as a liquid in 50% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. JNK2: A Negative Regulator of Cellular Proliferation. Sabapathy K, Wagner EF, Cell Cycle 2004 Dec 18;3(12) 2. c-Jun N-terminal protein kinase 1 (JNK1), but not JNK2, is essential for tumor necrosis factor alpha-induced c-Jun kinase activation and apopto sis. Liu J, Minemoto Y, Mol Cell Biol 2004 Dec;24(24):10844-56 3. Requirement of JNK2 for scavenger receptor A-mediated foam cell formation in atherogenesis. Ricci R, Sumara G, Rozenberg I, Eriksson U, Wagner EF, Science 2004 Nov 26;306(5701):1558-61 4. Hyperphosphorylation of extracellular regulated kinase 2 (ERK2) and inhibition of JNK2 phosphorylation are associated with increased S-phase during transformation of Syrian hamster embryo cells by Malachite Green. Bose B, Gour RR, Gupta S, Cell Biol Int 2004;28(12):875-83 5. The effect of C-terminal fragment of JNK2 on the stability of p53 and cell proliferation. Yin ZM, Sima J, Zhu J, Cell Res 2004 Oct;14(5):434-8 6. JNK2 translocates to the mitochondria and mediates cytochrome c release in PC12 cells in response to 6-hydroxydopamine. Eminel S, Klettner A, Herdegen T, J Biol Chem 2004 Dec 31;279(53):55385-92.