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K5 Heparan Lyase, Recombinant

Cat no: K0001-10

K5 Heparan Lyase, Recombinant

Heparan K5 Lyase is a recombinant enzyme. Each aliquot contains 100 milli-international units and 100ug total protein (92ug BSA as carrier) lyophilized from solution in 50mM Tris, 50mM NaCl, and 0.1% Triton X100, pH 8.0. The enzyme is feeze-dried in 100ul aliquots from.\nThe unit definition is the activity that releases 1 micromole of delta (4,5) hexuronate per minute at 37 degrees C using an extinction coefficient of 5100 per cm per M at 232nm for the delta (4,5) hexuronate product.\n\nSource:\nE. coli, strain TOP10, a disabled K-12 strain\n\nMolecular Weight:\n~52.3kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0001-10

Size

100mU

Form

White powder.

Purity

Purified (SDS-PAGE)

References

1. Murphy et al., (2004) J. Biol. Chem. 279, 27239-27245 2. Clarke et al, (2000) J Bacteriol. 182, 3716-3766.

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