Kallikrein 5 (KLK5) BioAssay(TM) ELISA Kit (Human)

Kallikrein 5 (KLK5) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK5 in human serum, plasma, tissue homogenates, saliva and other biological fluids.\n\nDetection Range:\n78-5000pg/ml.\n\nSensitivity:\n29pg/ml.\n\nIntra-Assay CV:\n< 10% \n\nInter-Assay CV:\n< 12%\n\nKit Components:\n*026335A: Microtiter Plate, 1x96wells, Pre-coated, ready to use\n*026335B: Standard, 2x1vial \n026335C: Standard Diluent, 1x20ml\n*026335D: Detection Reagent A, 1x120ul \n*026335E: Detection Reagent B, 1x120ul \n026335F: Assay Diluent A, 1x12ml\n026335G: Assay Diluent B, 1x12ml\n026335H: TMB Substrate, 1x9ml\n026335K: Stop Solution, 1x6ml\n026335L: Wash Buffer, 30X, 1x20ml \n\nPrecaution:\nThe Stop Solution (026335K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.\n\nStorage and Stability:\nStore *026335A, *026335B, *026335D and *026335E at -20 degrees C. Store all the other components at 4 degrees C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.\n\nProtocol Summary:\n1. Prepare all reagents, samples and standards; \n2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degrees C.\n3. Aspirate and add 100ul prepared Detection Reagent A immediately. Incubate 1 hour at 37 degrees C. \n4. Aspirate and wash 3 times. \n5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degrees C. \n6. Aspirate and wash 5 times.\n7. Add 90ul 026335H: TMB Substrate. Incubate 15-25 minutes at 37 degrees C. \n8. Add 50ul 026335K: Stop Solution. Read at 450nm immediately.

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