Kallistatin/Serpina4 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Kallistatin/Serpina4 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nSerpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, also called Kallistatin, is a protein that in humans is encoded by the SERPINA4 gene. This gene is mapped to chromosome 14q32.13. Kallistatin is a serine proteinase inhibitor (serpin) and a heparin-binding protein. It is localized in vascular smooth muscle cells and endothelial cells of blood vessels, suggesting that it may be involved in the regulation of vascular function. It shows that kallistatin plays a role in neointima hyperplasia. The potential role of kallistatin was investigated in angiogenesis in vitro and in vivo, and presented results demonstrating a novel role of the protein in the inhibition of angiogenesis and tumor growth.\n\nAssay Range:\n156pg/ml-10000pg/ml\n\nSensitivity:\n < 10 pg/ml \n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP29622\n\nEntrez Gene ID:\n5267\n\nKit Components:\n96-well plate pre-coated with anti-Kallistatin/Serpina4, Human\nLyophilized recombinant Kallistatin/Serpina4, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143938

Size

96Tests

References

1. Miao, R. Q., Agata, J., Chao, L., Chao, J. Kallistatin is a new inhibitor of angiogenesis and tumor growth. Blood 100: 3245-3252, 2002.\n2. Miao, R. Q., Murakami, H., Song, Q., Chao, L., Chao, J. Kallistatin stimulates vascular smooth muscle cell proliferation and migration in vitro and neointima formation in balloon-injured rat artery. Circ. Res. 86: 418-424, 2000.\n3. Zhou, G. X., Chao, L., Chao, J. Kallistatin: a novel human tissue kallikrein inhibitor; purification, characterization, and reactive center sequence. J. Biol. Chem. 267: 25873-25880, 1992.\n