Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.
Application:
Suitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.
Form: Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
pH:6.5-8.5
Appearance: Clear solution
Chromatographic Purity: To pass USP
Identity: To pass USP
Activity: (same/more than) 750ug/mg
Crystallinity: To pass USP
Specific Rotation: +107 degrees to +121 degrees
Endotoxin: (same/less than) 2Eu/mg
Kanamycin B Content (TLC): (same/less than) 4%
pH (Acidity or Alkalinity): 6.5-8.5
Sulfate: 15.0-17.0%
Sulfated Ash: (same/less than) 0.5%
Abnormal Toxicity: To pass BP98
Depressor Substance: To pass BP98
Working Concentration:
Cell/Tissue Culture: ~30ug/ml
Stringent Plasmids: 10ug/ml
Relaxed Plasmids: 50ug/ml
Storage and Stability:
Store liquid at 4 degrees C. Stable for for 12 months at 4 degrees C. Further dilutions can be made in assay buffer. Stability at 37 degrees C: 5 days.
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